Deep Eutectic Solvent-Based Microwave-Assisted Extraction for the Extraction of Seven Main Flavonoids from Ribes mandshuricum (Maxim.) Kom. Leaves

Flavonoids exhibit many biological properties, so it is very important to find an efficient and green method extract them from plant materials. In this paper, DES-MAE (deep eutectic solvent-based microwave-assisted extraction) technique was developed the seven major active flavonoids Ribes mandshuricum leaves, namely, trifolin, isoquercetin, rutin, astragalin, quercetin, hyperoside, kaempferol. After completion of extraction process, macroporous adsorption resin used for purification flavonoids. The BBD (Box–Behnken design) combined with RSM (response surface methodology) applied acquire optimal operating conditions DES-MAE. parameters were: temperature: 54 °C, time: 10 min, solvent: choline chloride/lactic acid a 1:2 mass ratio, water content: 25%, liquid/solid ratio: 27 mL/g. yields target were 4.78, 2.57, 1.25, 1.15, 0.34, 0.32, 0.093 mg/g DW (dry weight), respectively. direct kaempferol in solution achieved by using X-5. recoveries 87.02%, 81.37%, 79.64%, 87.13%, 97.36%, 88.08%, 99.39%, results showed that followed MRCC (macroporous column chromatography) represents promising approach extracting separating components plants.